Culture and regeneration of Lycopersicon peruvianum leaf protoplasts.
نویسندگان
چکیده
منابع مشابه
Regeneration from leaf protoplasts of Arabidopsis thaliana ecotype estland.
Protoplasts (2 x 10(7)/g fresh wt) were isolated from leaves of A. thaliana ecotype estland, with a viability of more than 90%. Protoplasts cultured in calcium alginate beads or layers showed division while culture in liquid or agarose beads failed to elicit any division. Effect of culture density showed highest frequency of division occurring at 5 x 10(5) while no division was seen when cultur...
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Protoplasts of Convolvulus arvensis L. tissue culture regenerated a wall-like structure within 3 days in culture. Although unusually electron dense and atypically amorphous in the electron microscope, this structure could be digested with Myrothecium cellulase but was resistant to protease, a Rohm and Haas pectinase, and a beta-1, 3-exoglucanase just like the original wall. A cytochemical test ...
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A finely dispersed cell suspension was obtained from embryogenic calli of two genotypes of pearl millet (Pennisetum americanum L. Mil cereal). Protoplasts isolated from both cell suspensions divided and produced globular embryoids and calli. On an agar medium, the embryoids were induced to form albino shoots or albino plantlet- like structures
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Self-incompatibility is one of the mechanisms that has evolved to encourage outbreeding in flowering plants (de Nettancourt, 1977). One particular type of self-incompatibility, gametophytic self-incompatibility, is found in members of Solanaceae, such as the wild tomato, Lycopersicon peruvianum (Mau et al., 1986). Gametophytic self-incompatibility in L. peruvianum is controlled by a single locu...
متن کاملIsolation and regeneration of protoplasts from Ectocarpus.
This article describes how to obtain isolated cells with no surrounding cell wall by enzymatic digestion of Ectocarpus filaments. The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions. The yield of protoplasts and their capacity to regenerate are highly dependent on the Ectocarpus strain used, the stage of the life cycle, and th...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1983
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.33.359